聯系方式 Contact

地址:廈門市集美區集美大道1300號

電話:15805933710

聯系人:許先生

QQ:1974707632

微信:15805933710

郵件:purimag@139.com

在線QQ交談 在線QQ交談

搜索 Search
你的位置:首頁 > 新聞動態 > 公司新聞

客戶采用我司鏈霉親和素磁珠在ACS Appl. Mater. Interfaces發文

2022-3-9 22:09:54點擊:

客戶采用我司鏈霉親和素磁珠(PuriMag G-strep)偶聯biotin修飾的糖基

Influenza Virus Precision Diagnosis and Continuous Purification Enabled by Neuraminidase-Resistant Glycopolymer-Coated Microbeads

Gefei Li, Wenxiao Ma, Juan Mo, Boyang Cheng, Shin-ichiro Shoda, Demin Zhou*, and Xin-Shan Ye*
Cite this: ACS Appl. Mater. Interfaces 2021, 13, 39, 46260–46269
Publication Date:September 22, 2021

https://doi.org/10.1021/acsami.1c11561

Abstract
Rapid diagnosis and vaccine development are critical to prevent the threat posed by viruses. However, rapid tests, such as colloidal gold assays, yield false-negative results due to the low quantities of viruses; moreover, conventional virus purification, including ultracentrifugation and nanofiltration, is multistep and time-consuming, which limits laboratory research and commercial development of viral vaccines. A rapid virus enrichment and purification technique will improve clinical diagnosis sensitivity and simplify vaccine production. Hence, we developed the surface-glycosylated microbeads (glycobeads) featuring chemically synthetic glycoclusters and reversible linkers to selectively capture the influenza virus. The surface plasmon resonance (SPR) evaluation indicated broad spectrum affinity of S-linked glycosides to various influenza viruses. The magnetic glycobeads were integrated into clinical rapid diagnosis, leading to a 30-fold lower limit of detection. Additionally, the captured viruses can be released under physiological conditions, delivering purified viruses with >50% recovery and without decreasing their native infectivity. Notably, this glycobead platform will facilitate the sensitive detection and continuous one-step purification of the target virus that contributes to future vaccine production.
快速診斷和疫苗開發對于預防病毒構成的威脅至關重要。然而,由于病毒數量少,快速檢測(如膠體金檢測)會產生假陰性結果;此外,包括超速離心和納濾在內的常規病毒純化是多步驟且耗時的,這限制了病毒疫苗的實驗室研究和商業開發??焖俨《靖患图兓夹g將提高臨床診斷的敏感性并簡化疫苗生產。因此,我們開發了具有化學合成糖簇和可逆接頭的表面糖基化微珠(糖珠),以選擇性地捕獲流感病毒。表面等離子共振 (SPR) 評估表明 S-連接糖苷對各種流感病毒具有廣譜親和力。磁性糖珠被整合到臨床快速診斷中,導致檢測下限降低 30 倍。此外,捕獲的病毒可以在生理條件下釋放,以> 50%的回收率提供純化的病毒,并且不會降低其天然傳染性。值得注意的是,這個糖珠平臺將促進目標病毒的靈敏檢測和連續一步純化,有助于未來的疫苗生產。


鏈霉親和素磁珠請參考 http://www.jsxwttc.com/Product/8271042221.html

亚洲中文无码永久免